IL18BP antibody (Biotin)

Catalog No. ABIN465095

This Goat Polyclonal antibody specifically detects IL18BP in WB and ELISA. It exhibits reactivity toward Mouse.

Quick Overview for IL18BP antibody (Biotin) (ABIN465095)

Target: IL18BP (Interleukin 18 Binding Protein (IL18BP))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This IL18BP antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-IL18BP Antibody (Biotin)

Specificity: Mouse IL-18BP

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine IL-18BP.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: ELISA: To detect mIL-18BP by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-18BP. To detect mIL-18BP by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IL-18BP (LS-C104411) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-18BP. Western Blot: To detect mIL-18BP by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-18BP is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL18BP

Target: IL18BP (Interleukin 18 Binding Protein (IL18BP))

Alternative Name: IL18BP

Background: Name/Gene ID: IL18BP
Family: Interleukin

Synonyms: IL18BP, IL18BPa, Interleukin-18-binding protein, Interleukin 18 binding protein, Tadekinig-alfa, IL-18BP

Gene ID: 10068

UniProt: O95998