IL16 antibody (Biotin)
Quick Overview for IL16 antibody (Biotin) (ABIN465160)
Target
See all IL16 AntibodiesReactivity
Host
Clonality
Conjugate
Application
-
-
Specificity
- Human IL-16
-
Purification
- Immunoaffinity purified
-
Immunogen
-
E.coli derived recombinant Human IL-16.
Type of Immunogen: Cell extract
-
-
-
-
Application Notes
-
Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)
Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hIL-16 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-16. To detect hIL-16 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-16 (LS-C104445) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-16. Western Blot: To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5-3 ng/lane, under either reducing or non-reducing conditions. -
Comment
-
Target Species of Antibody: Human
-
Restrictions
- For Research Use only
-
-
-
Format
- Lyophilized
-
Reconstitution
- Reconstitute with sterile PBS
-
Concentration
- Lot specific
-
Buffer
- Lyophilized from PBS, pH 7.2
-
Handling Advice
- Avoid freeze/thaw cycles.
-
Storage
- 4 °C,-20 °C
-
Storage Comment
-
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
-
-
- IL16 (Interleukin 16 (IL16))
-
Alternative Name
- IL16
-
Background
-
Name/Gene ID: IL16
Family: Hormone
Synonyms: IL16, IL-16, Interleukin 16, Neuronal interleukin 16, PrIL-16, NIL16, HsT19289, LCF, PRIL16, Pro-interleukin-16, Prointerleukin 16 -
Gene ID
- 3603
-
UniProt
- Q14005
Target
-