IL16 antibody (Biotin)

Catalog No. ABIN465160

The Biotin-conjugated Rabbit Polyclonal anti-IL16 antibody (ABIN465160) specifically detects IL16 in WB, ELISA and Neut. The antibody is reactive with Human samples.

Quick Overview for IL16 antibody (Biotin) (ABIN465160)

Target: IL16 (Interleukin 16 (IL16))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL16 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL16 Antibody (Biotin)

Specificity: Human IL-16

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-16.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hIL-16 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-16. To detect hIL-16 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-16 (LS-C104445) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-16. Western Blot: To detect hIL-16 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-16 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with sterile PBS

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL16

Target: IL16 (Interleukin 16 (IL16))

Alternative Name: IL16

Background: Name/Gene ID: IL16
Family: Hormone

Synonyms: IL16, IL-16, Interleukin 16, Neuronal interleukin 16, PrIL-16, NIL16, HsT19289, LCF, PRIL16, Pro-interleukin-16, Prointerleukin 16

Gene ID: 3603

UniProt: Q14005