CXCL14 antibody

Catalog No. ABIN465239

The Rabbit Polyclonal anti-CXCL14 antibody has been validated for ELISA and WB. It is suitable to detect CXCL14 in samples from Human.

Quick Overview for CXCL14 antibody (ABIN465239)

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCL14 antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-CXCL14 Antibody

Specificity: Human BRAK

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human BRAK.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.25 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hBRAK by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human BRAK (LS-C104495) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hBRAK. Western Blot: To detect hBRAK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hBRAK is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CXCL14

Target: CXCL14 (Chemokine (C-X-C Motif) Ligand 14 (CXCL14))

Alternative Name: CXCL14

Background: Name/Gene ID: CXCL14
Subfamily: Intercrine alpha
Family: Intercrine

Synonyms: CXCL14, BRAK, C-X-C motif chemokine 14, Bolekine, Breast and kidney, Chemokine BRAK, KS1, Mip2 gamma, MIP2G, SCYB14, Small-inducible cytokine B14, MIP-2 gamma, BMAC, Tumor-suppressing chemokine, KEC, MIP-2g, NJAC

Gene ID: 9547

UniProt: O95715

Pathways: Autophagy