IL1A antibody (Biotin)

Catalog No. ABIN465287

This Rabbit Polyclonal antibody specifically detects IL1A in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for IL1A antibody (Biotin) (ABIN465287)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL1A antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL1A Antibody (Biotin)

Specificity: Human IL-1alpha

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human IL-1alpha.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hIL-1alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-1alpha. To detect hIL-1alpha by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human IL-1alpha (LS-C104630) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hIL-1alpha. Western Blot: To detect hIL-1alpha by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-1alpha is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Alternative Name: IL1A / IL1 Alpha

Background: Name/Gene ID: IL1A
Family: IL-1

Synonyms: IL1A, Hematopoietin-1, IL-1 alpha, IL1, IL1-ALPHA, IL1 Alpha, Interleukin-1 alpha, IL-1A, IL1F1, Pro-interleukin-1-alpha, Preinterleukin 1 alpha, Interleukin 1, alpha

Gene ID: 3552

UniProt: P01583

Pathways: NF-kappaB Signaling, Autophagy, Cancer Immune Checkpoints