PLGF antibody (Biotin)
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- Target See all PLGF (PGF) Antibodies
- PLGF (PGF) (Placenta Growth Factor (PGF))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PLGF antibody is conjugated to Biotin
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Application
- Western Blotting (WB), ELISA
- Specificity
- Human PlGF-1
- Purification
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human PlGF-1.
Type of Immunogen: Cell extract - Top Product
- Discover our top product PGF Primary Antibody
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- Application Notes
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Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)
Usage: ELISA: To detect hPlGF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hPlGF. To detect hPlGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human PlGF as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hPlGF. Western Blot: To detect hPlGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPlGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile PBS containing 0.1 % BSA
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- PLGF (PGF) (Placenta Growth Factor (PGF))
- Alternative Name
- PGF / PLGF (PGF Products)
- Synonyms
- D12S1900 antibody, PGFL antibody, PLGF antibody, PlGF-2 antibody, SHGC-10760 antibody, AI854365 antibody, PIGF antibody, Plgf antibody, PlGF antibody, placental growth factor antibody, placenta growth factor antibody, PGF antibody, Pgf antibody, PLGF antibody
- Background
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Name/Gene ID: PGF
Family: PDGF
Synonyms: PGF, D12S1900, PGFL, PlGF-2, Placental growth factor, Placental growth factor-like, PLGF, Placenta growth factor, SHGC-10760 - Gene ID
- 5228
- UniProt
- P49763
- Pathways
- VEGFR1 Specific Signals
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