CCL7 antibody (Biotin)

Catalog No. ABIN465392

This Goat Polyclonal antibody specifically detects CCL7 in WB, ELISA and Neut. It exhibits reactivity toward Mouse.

Quick Overview for CCL7 antibody (Biotin) (ABIN465392)

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This CCL7 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CCL7 Antibody (Biotin)

Specificity: Mouse MCP-3

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine MCP-3.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect mMCP-3 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mMCP-3. To detect mMCP-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine MCP-3 (LS-C104607) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mMCP-3. Western Blot: To detect mMCP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mMCP-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CCL7

Target: CCL7 (Chemokine (C-C Motif) Ligand 7 (CCL7))

Alternative Name: CCL7 / MCP-3

Background: Name/Gene ID: CCL7
Subfamily: Intercrine beta
Family: Intercrine

Synonyms: CCL7, C-C motif chemokine 7, Chemokine (C-C motif) ligand 7, FIC, MARC, MCP3, Monocyte chemotactic protein 3, SCYA7, Small-inducible cytokine A7, MCP-3, SCYA6, NC28

Gene ID: 6354

UniProt: P80098