RETNLB antibody

Catalog No. ABIN465494

Product Details anti-RETNLB Antibody

Target: RETNLB (Resistin Like beta (RETNLB))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RETNLB antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Mouse RELMbeta

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine RELMbeta.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA, WB (0.1 - 0.2 μg/mL)

Usage: Immunohistochemistry: This antibody stained 4 % PFA, paraffin-embedded sections of murine cecum tissue (with positive goblet cells of a Trichuris muris infected mouse). The primary antibody was incubated at a concentration of 0.33 ng/mL overnight at 4 °C and the secondary antibody was a cyanine-2 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100  mM Citric Acid was used. Information and photos are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary. ELISA: To detect Murine RELMbeta by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine RELMbeta (LS-C104626) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine RELMbeta. Western Blot: To detect Murine RELMbeta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. when used in conjunction with compatible secondary reagents, the detection limit for recombinant Murine RELMbeta is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

References for anti-RETNLB antibody (ABIN465494)

Varyani, Löser, Filbey, Harcus, Drurey, Poveda, Rasid, White, Smyth, Gerbe, Jay, Maizels: "The IL-25-dependent tuft cell circuit driven by intestinal helminths requires macrophage migration inhibitory factor (MIF)." in: Mucosal immunology, (2022) (PubMed).

Target Details for RETNLB

Target: RETNLB (Resistin Like beta (RETNLB))

Alternative Name: FIZZ1 / RELM-Beta (RETNLB Products)

Synonyms: FIZZ1 antibody, FIZZ2 antibody, HXCP2 antibody, RELM-beta antibody, RELMb antibody, RELMbeta antibody, XCP2 antibody, RETNLB antibody, 9030012B21Rik antibody, Fizz2 antibody, Relmb antibody, Xcp3 antibody, resistin like beta antibody, RETNLB antibody, Retnlb antibody

Background: Name/Gene ID: RETNLB

Synonyms: RETNLB, CCRG, Fizz1, HXCP2, Found in inflammatory zone 1, Resistin like beta, Resistin-like beta, RELM-beta, RELMb, RELMbeta, RETNL2, FIZZ2, Xcp2

Gene ID: 84666

UniProt: Q9BQ08

Pathways: Hormone Activity