Thrombopoietin antibody (Biotin)

Catalog No. ABIN465560

This Goat Polyclonal antibody specifically detects Thrombopoietin in WB, ELISA and Neut. It exhibits reactivity toward Human.

Quick Overview for Thrombopoietin antibody (Biotin) (ABIN465560)

Target: Thrombopoietin (THPO)

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Thrombopoietin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-Thrombopoietin Antibody (Biotin)

Specificity: Human TPO

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TPO.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hTPO by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO. To detect hTPO by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TPO (LS-C104802) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTPO. Western Blot: To detect hTPO by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTPO is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Thrombopoietin

Target: Thrombopoietin (THPO)

Alternative Name: THPO / TPO / Thrombopoietin

Background: Name/Gene ID: THPO
Family: Hormone

Synonyms: THPO, C-mpl ligand, MGDF, MPL ligand, THCYT1, Thrombopoietin nirs variant 1, MKCSF, MPLLG, Thrombopoietin

Gene ID: 7066

UniProt: P40225

Pathways: JAK-STAT Signaling, Hormone Activity