KIT Ligand antibody

Catalog No. ABIN465573

This anti-KIT Ligand antibody is a Rabbit Polyclonal antibody detecting KIT Ligand in WB, ELISA and Neut. Suitable for Human.

Quick Overview for KIT Ligand antibody (ABIN465573)

Target: KIT Ligand (KITLG)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This KIT Ligand antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-KIT Ligand Antibody

Specificity: Human SCF

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human SCF.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (8 - 12 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 0.5 μg/mL-1 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Optimal results for these conditions were achieved with enzyme induced antigen retrieval (proteinase K) at room temperature. Optimal concentrations and conditions may vary. *Additional Immunostaining data available. Please contact Tech Support for information. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hSCF (10.00 ng/mL), a concentration of 0.64-0.96 μg/mL of this antibody is required. ELISA: To detect hSCF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human SCF (LS-C104672) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hSCF. Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for KIT Ligand

Target: KIT Ligand (KITLG)

Alternative Name: KITLG / SCF

Background: Name/Gene ID: KITLG

Synonyms: KITLG, C-Kit ligand, KIT ligand, Kitl, KL-1, SHEP7, SF, Steel factor, Stem cell factor, SCF, FPH2, Mast cell growth factor, MGF

Gene ID: 4254

UniProt: P21583

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway