Thymic Stromal Lymphopoietin antibody (Biotin)

Catalog No. ABIN465619

The Biotin-conjugated Rabbit Polyclonal anti-Thymic Stromal Lymphopoietin antibody (ABIN465619) specifically detects Thymic Stromal Lymphopoietin in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for Thymic Stromal Lymphopoietin antibody (Biotin) (ABIN465619)

Target: Thymic Stromal Lymphopoietin (TSLP)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Thymic Stromal Lymphopoietin antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Product Details anti-Thymic Stromal Lymphopoietin Antibody (Biotin)

Specificity: Human TSLP

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human TSLP.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hTSLP by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTSLP. To detect hTSLP by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TSLP (LS-C104691) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTSLP. Western Blot: To detect hTSLP by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTSLP is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Thymic Stromal Lymphopoietin

Target: Thymic Stromal Lymphopoietin (TSLP)

Alternative Name: TSLP

Background: Name/Gene ID: TSLP
Family: Cytokine

Synonyms: TSLP, Thymic stromal lymphopoietin

Gene ID: 85480

UniProt: Q969D9