FGF7 antibody

Catalog No. ABIN465624

This anti-FGF7 antibody is a Rabbit Polyclonal antibody detecting FGF7 in WB, ELISA and Neut. Suitable for Human.

Quick Overview for FGF7 antibody (ABIN465624)

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF7 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-FGF7 Antibody

Specificity: Human KGF

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human KGF.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.56 - 0.85 μg/mL), WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of normal human skin. The recommended concentration is 0.5 μg/mL overnight at 4 °C. An HRP-labeled polymer detection system was used with a non-alcohol soluble AEC chromogen and a proteinase K antigen retrieval. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND]of the biological activity of hKGF (25 ng/mL), a concentration of 0.56-0.85 μg/mL of this antibody is required. ELISA: To detect hKGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human KGF (LS-C104287) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hKGF. Western Blot: To detect hKGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hKGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for FGF7

Target: FGF7 (Fibroblast Growth Factor 7 (FGF7))

Alternative Name: FGF7 / KGF

Background: Name/Gene ID: FGF7
Family: HBGF

Synonyms: FGF7, FGF-7, Fibroblast growth factor- 7, Keratinocyte growth factor, Fibroblast growth factor 7, HBGF-7, KGF

Gene ID: 2252

UniProt: P21781

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway