Cardiotrophin 1 antibody (Biotin)

Catalog No. ABIN465627

The Rabbit Polyclonal anti-Cardiotrophin 1 antibody has been validated for WB, ELISA and Neut. It is suitable to detect Cardiotrophin 1 in samples from Human.

Quick Overview for Cardiotrophin 1 antibody (Biotin) (ABIN465627)

Target: Cardiotrophin 1 (CTF1)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cardiotrophin 1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-Cardiotrophin 1 Antibody (Biotin)

Specificity: Human Cardiotrophin-1

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human Cardiotrophin-1.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hCardiotrophin-1 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hCardiotrophin-1. To detect hCardiotrophin-1 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human Cardiotrophin-1(LS-C104623) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hCardiotrophin-1. Western Blot: To detect hCardiotrophin-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCardiotrophin-1 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for Cardiotrophin 1

Target: Cardiotrophin 1 (CTF1)

Alternative Name: CFT1 / CT-1 / Cardiotrophin-1

Background: Name/Gene ID: CTF1

Synonyms: CTF1, Cardiotrophin 1, Cardiotrophin-1, CT1, Cardiophin 1, CT-1

Gene ID: 1489

UniProt: Q16619

Pathways: JAK-STAT Signaling