CNTF antibody (Biotin)

Catalog No. ABIN465710

This Rabbit Polyclonal antibody specifically detects CNTF in WB, ELISA and Neut. It exhibits reactivity toward Rat.

Quick Overview for CNTF antibody (Biotin) (ABIN465710)

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNTF antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-CNTF Antibody (Biotin)

Specificity: Rat CNTF

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Rat CNTF.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Rat CNTF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. To detect Rat CNTF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Rat CNTF (LS-C104756) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat CNTF. Western Blot: To detect Rat CNTF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat CNTF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for CNTF

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Alternative Name: CNTF

Background: Name/Gene ID: CNTF

Synonyms: CNTF, Ciliary neurotrophic factor, HCNTF

Gene ID: 1270

UniProt: P26441

Pathways: JAK-STAT Signaling