IL-7 antibody (Biotin)

Catalog No. ABIN465836

This Rabbit Polyclonal antibody specifically detects IL-7 in WB, ELISA and Neut. It exhibits reactivity toward Mouse.

Quick Overview for IL-7 antibody (Biotin) (ABIN465836)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-7 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL-7 Antibody (Biotin)

Specificity: Mouse IL-7

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine IL-7.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect Murine IL-7 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IL-7. To detect Murine IL-7 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine IL-7 (LS-C104812) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine IL-7. Western Blot: To detect Murine IL-7 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant Murine IL-7 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile PBS containing 0.1 % BSA

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Alternative Name: IL7

Background: Name/Gene ID: IL7
Family: IL-7/ IL-9

Synonyms: IL7, IL-7, Interleukin-7, Interleukin 7

Gene ID: 3574

UniProt: P13232

Pathways: JAK-STAT Signaling