IL-3 antibody

Catalog No. ABIN465873

This anti-IL-3 antibody is a Rabbit Polyclonal antibody detecting IL-3 in WB, ELISA and Neut. Suitable for Mouse.

Quick Overview for IL-3 antibody (ABIN465873)

Target: IL-3 (Interleukin 3 (IL-3))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Neutralization (Neut)

Product Details anti-IL-3 Antibody

Specificity: Mouse IL-3

Purification: Immunoaffinity purified

Immunogen: E.coli derived recombinant Murine IL-3.

Type of Immunogen: Cell extract

Application Details

Application Notes: Approved: ELISA (0.5 - 2 μg/mL), Neut (0.15 - 0.2 μg/mL), WB

Usage: Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of mIL-3 (0.5 ng/mL), a concentration of 0.15-0.20 μg/mL of this antibody is required. ELISA: To detect mIL-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-3 (LS-C104814) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-3. Western Blot: To detect mIL-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-3 is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Sterile water

Concentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handling Advice: Avoid freeze/thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Target Details for IL-3

Target: IL-3 (Interleukin 3 (IL-3))

Alternative Name: IL3

Background: Name/Gene ID: IL3
Family: Hormone

Synonyms: IL3, Hematopoietic growth factor, IL-3, Mast-cell growth factor, MULTI-CSF, P-cell-stimulating factor, Interleukin-3, Mast cell growth factor, MCGF, P-cell stimulating factor

Gene ID: 3562

UniProt: P08700

Pathways: JAK-STAT Signaling, Regulation of Carbohydrate Metabolic Process, Autophagy