CXCL9 antibody
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- Target See all CXCL9 Antibodies
- CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This CXCL9 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Neutralization (Neut)
- Specificity
- Human MIG
- Purification
- Immunoaffinity purified
- Immunogen
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E.coli derived recombinant Human MIG.
Type of Immunogen: Cell extract - Top Product
- Discover our top product CXCL9 Primary Antibody
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- Application Notes
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Approved: ELISA (0.5 - 2 μg/mL), Neut (5 - 7 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human renal tumor with parenchyma tissue. The recommended concentration is 0.25 μg/mL-0.50 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Optimal results for these conditions were achieved with heat induced antigen retrieval with a pH 6 sodium citrate buffer. Optimal concentrations and conditions may vary. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of hMIG (100 ng/mL), a concentration of 5-7 μg/mL of this antibody is required. ELISA: To detect hMIG by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MIG (LS-C104825) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hMIG. Western Blot: To detect hMIG by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMIG is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Sterile water
- Concentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handling Advice
- Avoid freeze/thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- Target
- CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))
- Alternative Name
- MIG / CXCL9 (CXCL9 Products)
- Synonyms
- CMK antibody, Humig antibody, MIG antibody, SCYB9 antibody, crg-10 antibody, BB139920 antibody, Mig antibody, MuMIG antibody, Scyb9 antibody, C-X-C motif chemokine ligand 9 antibody, chemokine (C-X-C motif) ligand 9 antibody, CXCL9 antibody, Cxcl9 antibody
- Background
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Name/Gene ID: CXCL9
Subfamily: Intercrine alpha
Family: Intercrine
Synonyms: CXCL9, Crg-10, Humig, SCYB9, Small-inducible cytokine B9, C-X-C motif chemokine 9, CMK, MIG - Gene ID
- 4283
- UniProt
- Q07325
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