HMGB1 antibody (AA 1-216)

Catalog No. ABIN466031

The Mouse Monoclonal anti-HMGB1 antibody has been validated for WB and IF. It is suitable to detect HMGB1 in samples from Human.

Quick Overview for HMGB1 antibody (AA 1-216) (ABIN466031)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HMGB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 1E6-E10

Product Details anti-HMGB1 Antibody

Binding Specificity: AA 1-216

Purification: Purified from ascites by Protein A

Immunogen: HMGB1 (AAH03378, 1 a.a. ~ 216 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

Type of Immunogen: Recombinant protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: IF (10 μg/mL), WB

Usage: Western Blot (Transfected lysate) - positive control transfected 293T cell line. Immunofluorescence (10 μg/mL) - positive control HeLa cells.

Not recommended for: IHC-P

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.4

Handling Advice: Aliquot to Avoid freeze/thaw cycles.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Target Details for HMGB1

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: HMG1 / HMGB1

Background: Name/Gene ID: HMGB1

Synonyms: HMGB1, Amphoterin, High mobility group protein B1, High mobility group box 1, High mobility group protein 1, HMG1, HMG3, SBP-1, High-mobility group box 1, HMG-1

Gene ID: 3146

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome