FZD4 antibody (Cysteine-Rich Domain, Extracellular)

Catalog No. ABIN478754

The Goat Polyclonal anti-FZD4 antibody has been validated for WB, ELISA, IHC and ICC. It is suitable to detect FZD4 in samples from Mouse.

Quick Overview for FZD4 antibody (Cysteine-Rich Domain, Extracellular) (ABIN478754)

Target: FZD4 (Frizzled Family Receptor 4 (FZD4))

Reactivity: Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This FZD4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunocytochemistry (ICC)

Product Details anti-FZD4 Antibody

Binding Specificity: Cysteine-Rich Domain, Extracellular

Specificity: Recognizes mouse Frizzled-4 extracellular Cysteine-Rich Domain (CRD). ~5 % cross-reactivity with rmFrizzled-8 and less than 2 % cross-reactivity with rmFrizzled-3 and rmFrizzled-7.

Purification: Immunoaffinity purified

Immunogen: NS0-derived rmFrizzled-4 extracellular Cysteine-Rich Domain (CRD).

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (0.5 - 1 μg/mL), ICC, IHC (15 μg/mL), WB (0.1 - 0.2 μg/mL)

Usage: Suitable for use in Direct ELISA, Western blot, Immunohistochemistry and Immunocytochemistry. Direct ELISA: 0.5-1 μg/mL. Western blot: 0.1-0.2 μg/mL The detection limit for rmFrizzled-4 is approximately 2 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: 15 μg/mL in fixed cells or tissues.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 40 % glycerol.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for FZD4

Target: FZD4 (Frizzled Family Receptor 4 (FZD4))

Alternative Name: FZD4 / Frizzled 4

Background: Name/Gene ID: FZD4
Subfamily: Frizzled
Family: GPCR

Synonyms: FZD4, CD344, CD344 antigen, EVR1, Frizzled family receptor 4, Frizzled-4, Fz-4, Fz4, HFz4, Exudative vitreoretinopathy 1, FEVR, FZD4S, FzE4, Frizzled 4, Frizzled homolog 4, WNT receptor frizzled-4

Gene ID: 8322

UniProt: Q9ULV1

Pathways: WNT Signaling, Hormone Transport, Sensory Perception of Sound