A2B5 antibody

Catalog No. ABIN479509

Product Details

Target: A2B5

Reactivity: Chicken, Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)

Specificity: Recognizes chicken Neuron Cell Surface Antigen A2B5. Reacts with a surface antigen on neurons in the retina, brain, spinal cord and dorsal root ganglia and with all tested human neuroblastoma cell lines. Does not seem to bind with any leukemic cell lines or with bone marrow cells from patients with leukemia, nor with cells from normal bone marrow. Cytotoxic to neurons in the presence of guinea-pig complement. Protein A binding is positive. Species cross-reactivity: human.

Purification: Purified

Immunogen: Chick retina cells.

Type of Immunogen: Cells

Isotype: IgM

Application Details

Application Notes: Approved: Flo, ICC (1:100 - 1:500), IF (1:100 - 1:500), IHC, IHC-Fr

Usage: Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry and Immunocytochemistry. Indirect Immunofluorescence: 1:100-1:500. For the detection of neurons in tissue culture. Using double immunofluorescence the GQ ganglioside has a similar distribution on neurons to the D2 protein, to the tetanus toxin receptor and to neurofilaments. Immunohistochemistry: Frozen, fixed tissues. Immunocytochemistry: Confocal fluorescent analysis of PC12 cells. Positive control: Type II astrocytes, human neural progenitors, PC12 cells.

Comment:

Target Species of Antibody: Chicken

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.1 % sodium azide, 40 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details

Target: A2B5

Alternative Name: Neuron Cell Surface Antigen A2b5