Nitrotyrosine antibody

Catalog No. ABIN479556

This anti-Nitrotyrosine antibody is a Mouse Monoclonal antibody detecting Nitrotyrosine in WB, IP and ICC. Suitable for .

Quick Overview for Nitrotyrosine antibody (ABIN479556)

Target: Nitrotyrosine

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This Nitrotyrosine antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-Nitrotyrosine Antibody

Specificity: Recognizes nitrated proteins from all species.

Purification: Protein G purified

Immunogen: Purified, nitrated Keyhole Limpet Hemocyanin (KLH).

Type of Immunogen: Hapten

Isotype: IgG2b kappa

Application Details

Application Notes: Approved: ICC (5 - 10 μg/mL), IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry. Western Blot: 0.5-2 μg/mL detects nitrotyrosine immunoblotting controls. A previous lot detected nitrated proteins in a lysate prepared from peroxynitrite-treated A431 cells. No reaction was noted with non-nitrated proteins. Immunoprecipitation: To be determined by the researcher. Immunocytochemistry: 5-10 μg/mL showed positive immunostaining for nitrated proteins in peroxynitrite-treated A431 cells fixed with 95 % ethanol/5 % acetic acid.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.4, 0.15 M sodium chloride, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Target Details for Nitrotyrosine

Target: Nitrotyrosine

Target Type: Chemical