S100A8/A9 Complex (Calprotectin) antibody

Catalog No. ABIN481651

This Mouse Monoclonal antibody specifically detects S100A8/A9 Complex (Calprotectin) in IHC, ELISA and IHC (p). It exhibits reactivity toward Human.

Quick Overview for S100A8/A9 Complex (Calprotectin) antibody (ABIN481651)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8/A9 Complex (Calprotectin) antibody is un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 27E10

Product Details anti-S100A8/A9 Complex (Calprotectin) Antibody

Specificity: The antigen is MRP8/14 (calprotectin), the epitope involves parts of both subunits MRP8 and MRP14. Antigen distribution: Isolated cells: Monocytes carry the antigen both on the surface and intracellularly, granulocytes exhibit it only intracellularly. Up to 80 % of monocytes in early cultures (24- 48h) are positive. No reaction has been seen with lymphocytes or platelets. Tissue sections: The antigen is found in macrophages in the red pulp of the spleen and liver. It is strongly expressed in macrophages from acute inflamed tissues (peridontitis, contact eczema, urticaria, erythrodermia) where some endothelial and epidermal cells may also express this antigen. It is normally absent on resident mononuclear phagocytes in healthy tissues (skin, gut, thymus). Specificity: Human: subpopulation of macrophages, monocytes and granulocytes, peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Other: subpopulation of macrophages of rhesus monkey and bovine tissues. The antibody does not react with swine tissues.

Purification: Affinity purified

Immunogen: Cultured human monocytes

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P (1 μg/mL)

Usage: For IHC-P, Proteinase K pretreatment is recommended for antigen retrieval.

Not recommended for: IHC-Fr

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS  pH 7.2, 5 mg/mL BSA, and 0.05 % Kathon CG

Preservative: Kathon CG

Handling Advice: Avoid freeze/thaw cycles. Store undiluted.

Storage: 4 °C,-20 °C

Storage Comment: Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.

Target Details for S100A8/A9 Complex (Calprotectin)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Alternative Name: Calprotectin

Pathways: S100 Proteins