S100A8/A9 Complex (Calprotectin) antibody (Biotin)

Catalog No. ABIN481652

The Mouse Monoclonal anti-S100A8/A9 Complex (Calprotectin) antibody has been validated for IHC, ELISA and IHC (p). It is suitable to detect S100A8/A9 Complex (Calprotectin) in samples from Human.

Quick Overview for S100A8/A9 Complex (Calprotectin) antibody (Biotin) (ABIN481652)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This S100A8/A9 Complex (Calprotectin) antibody is conjugated to Biotin

Application: Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 27E10

Product Details anti-S100A8/A9 Complex (Calprotectin) Antibody (Biotin)

Specificity: The antigen is MRP8/14 (calprotectin), the epitope involves parts of both subunits MRP8 and MRP14. Antigen distribution: Isolated cells: Monocytes carry the antigen both on the surface and intracellularly, granulocytes exhibit it only intracellularly. Up to 80 % of monocytes in early cultures (24- 48h) are positive. No reaction has been seen with lymphocytes or platelets. Tissue sections: The antigen is found in macrophages in the red pulp of the spleen and liver. It is strongly expressed in macrophages from acute inflamed tissues (peridontitis, contact eczema, urticaria, erythrodermia) where some endothelial and epidermal cells may also express this antigen. It is normally absent on resident mononuclear phagocytes in healthy tissues (skin, gut, thymus). Specificity: Human: subpopulation of macrophages, monocytes and granulocytes, peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. Other: subpopulation of macrophages of rhesus monkey and bovine tissues. The antibody does not react with swine tissues.

Purification: Affinity purified

Immunogen: Cultured human monocytes

Application Details

Application Notes: Approved: ELISA, IHC, IHC-P

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.5 mL distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS  pH 7.2, 5 mg/mL BSA, and 0.05 % Kathon CG

Preservative: Kathon CG

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details for S100A8/A9 Complex (Calprotectin)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Alternative Name: Calprotectin

Pathways: S100 Proteins