Macrophages antibody (Biotin)

Catalog No. ABIN481686

The Biotin-conjugated Rat Monoclonal anti-Macrophages antibody (Clone MOMA-1) (ABIN481686) specifically detects Macrophages in IHC (fro) and IHC. The antibody is reactive with Mouse samples.

Quick Overview for Macrophages antibody (Biotin) (ABIN481686)

Target: Macrophages

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: Biotin

Application: Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)

Clone: MOMA-1

Product Details

Specificity: The antigen is found intracellularly and on cell surface on metallophilic macrophages. Antigen distribution: Isolated cells: No reactivity of MOMA-1 was found with dendritic cells, peritoneal resident macrophages, peritoneal exudate cells, bone marrow or blood cells. Tissue sections: Distinct macrophage subpopulations of lymphoid organs express the antigen. In the spleen, they are localized at the marginal sinus forming a ring around the periarteriolar lymphocyte sheath and follicular areas at the inner side of marginal zones. In lymph nodes, they are localized in the sinusoids and medullary cords, but not within follicular areas or paracortex. In Peyer's patches they are localized in the interfollicular areas at the serosal side. Kupffer cells in the liver can be clearly stained by MOMA-1. No MOMA- 1-positive macrophages were found in the thymus, brain, kidney, liver, skin or heart. In non-lymphoid organs, the antigen is only found on a macrophage subpopulation in the lamina propria of the villi of the small intestine. Specificity: Mouse: Subpopulation of mature resident tissue macrophages. Other species: not tested.

Purification: Affinity purified

Immunogen: Lymph node

Application Details

Application Notes: Approved: IHC, IHC-Fr (1:400 - 1:800)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 0.5 mL distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS  pH 7.2, 5 mg/mL BSA, 0.05 % Kathon

Preservative: Kathon CG

Storage: 4 °C

Storage Comment: Store at 4°C.

Target Details

Target: Macrophages