LY9 antibody

Catalog No. ABIN487455

The Mouse Monoclonal anti-LY9 antibody (Clone HLy9-1-25) (ABIN487455) specifically detects LY9 in FACS. The antibody is reactive with Human samples.

Quick Overview for LY9 antibody (ABIN487455)

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LY9 antibody is un-conjugated

Application: Flow Cytometry (FACS)

Clone: HLy9-1-25

Product Details anti-LY9 Antibody

Specificity: This antibody reacts with Human Ly-9 antigen.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: Ly-9, T-lymphocyte surface antigen Ly-9, Lymphocyte antigen 9

Immunogen: Mouse pre-B-cell line 300. 19, stably transfected with the full length Human Ly-9 cDNA. Remarks: This antibody was concentrated from Hybridoma (clone HLy-9. 1. 25) supernatant. Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/c mousesplenocyte.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 10 μg/mLPositive Control: Peripheral blood T cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Flow Cytometric Analysis for Floating CellsWe use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 7.2 containing 50 % Glycerol without preservatives.

Preservative: Without preservative

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for LY9

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Alternative Name: CD229 / LY9

Background: CD229 is a 100-120 kD cell surface glycoprotein that is the homologue of murine Ly-9. CD229 is a member of the immunoglobulin superfamily and may have a function in cell signalling. The antigen is expressed at low levels by mature T and B lymphocytes and at higher levels by single-positive thymocytes. Expression at variable levels is also seen in some cell lines, such as Daudi, U937 and HUT78.Synonyms: Ly-9, Lymphocyte antigen 9, T-lymphocyte surface antigen Ly-9

Gene ID: 4063

UniProt: Q9HBG7

Pathways: BCR Signaling