LY9 antibody (FITC)

Catalog No. ABIN487456

This Mouse Monoclonal antibody specifically detects LY9 in FACS. It exhibits reactivity toward Human.

Quick Overview for LY9 antibody (FITC) (ABIN487456)

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LY9 antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: HLy9-1-25

Product Details anti-LY9 Antibody (FITC)

Specificity: This antibody reacts with Human Ly-9 antigen.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Characteristics: Synonyms: Ly-9, T-lymphocyte surface antigen Ly-9, Lymphocyte antigen 9

Purification: Protein-A Sepharose Chromatography.

Immunogen: Mouse pre-B-cell line 300. 19, stably transfected with the full length Human Ly-9 cDNA. Remarks: Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/cmouse splenocyte.

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 10 μg/mL (final concentration). Positive Control: Peripheral blood T cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Flow Cytometric Analysis for Floating CellsProtocol 1We use Fisher tubes or equivalents as reaction tubes for all step described below. 1) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS) and0. 1% BaN3]2) Add 10 µL of normal goat serum to the cell pellet after tapping. Mix well, and incubatefor 5 minutes at room temperature (20~25°C). 3) Add 30 µL of the FITC labeled CD229 monoclonal antibody (10-20 µg/mL) diluted withPBS containing 2% FCS. Mix well, and incubate for 30 minutes at room temperature(20~25°C). 4) Add 1 mL of the washing buffer followed by centrifugation at 500xg for 1 minute at RT(20~25°C). Remove supernatant by careful aspiration. 5) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytomete.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.05 mg/mL

Buffer: PBS, 7.2 containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for LY9

Target: LY9 (CD229) (Lymphocyte Antigen 9 (CD229))

Alternative Name: CD229 / LY9

Background: CD229 is a 100-120 kD cell surface glycoprotein that is the homologue of murine Ly-9. CD229 is a member of the immunoglobulin superfamily and may have a function in cell signalling. The antigen is expressed at low levels by mature T and B lymphocytes and at higher levels by single-positive thymocytes. Expression at variable levels is also seen in some cell lines, such as Daudi, U937 and HUT78.Synonyms: Ly-9, Lymphocyte antigen 9, T-lymphocyte surface antigen Ly-9

Gene ID: 4063

UniProt: Q9HBG7

Pathways: BCR Signaling