SERPINB5 antibody

Catalog No. ABIN487960

This anti-SERPINB5 antibody is a Rabbit Polyclonal antibody detecting SERPINB5 in WB and EIA. Suitable for Human.

Quick Overview for SERPINB5 antibody (ABIN487960)

Target: SERPINB5 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SERPINB5 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-SERPINB5 Antibody

Specificity: This antibody recognizes Maspin/SERPINB5.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography employing an immobilized Human Maspin matrix.

Immunogen: Highly purified recombinant Human Maspin.

Application Details

Application Notes: Sandwich Elisa: To detect Human Maspin by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with Biotinylated Anti-Human Maspin (ABIN487957 orABIN487958) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human Maspin. Western Blot: To detect Human Maspin by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondaryreagents the detection limit for recombinant Human Maspin is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody prior to reconstitution at-20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at-20 °C for longer.

Target Details for SERPINB5

Target: SERPINB5 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5))

Alternative Name: SERPINB5 / Maspin

Background: MASPIN is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, it exhibits no serine protease inhibitory activity.Synonyms: PI-5, PI5, Peptidase inhibitor 5, Protease inhibitor 5, Serpin B5

Gene ID: 5268

NCBI Accession: NP_002630

UniProt: P36952

Pathways: p53 Signaling