SERPINB5 antibody (AA 1-350)

Catalog No. ABIN3043330

Product Details anti-SERPINB5 Antibody

Target: SERPINB5 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5))

Binding Specificity: AA 1-350

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SERPINB5 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Purpose: Rabbit IgG polyclonal antibody for Serpin B5(SERPINB5) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Serpin B5(SERPINB5) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: serpin peptidase inhibitor, clade B (ovalbumin), member 5
Protein Name: Serpin B5

Purification: Immunogen affinity purified.

Immunogen: E.coli-derived human MASPIN recombinant protein (Position: M1-A350). Human MASPIN shares 88% and 89% amino acid (aa) sequence identity with mouse and rat MASPIN, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat, The detection limit for MASPIN is approximately 0.1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

References for anti-SERPINB5 antibody (ABIN3043330)

Zhang, Qi, Cao, Zu, Chen: "Expression and clinical significance of microRNA-21, maspin and vascular endothelial growth factor-C in bladder cancer." in: Oncology letters, Vol. 10, Issue 4, pp. 2610-2616, (2015) (PubMed).

Yang, Shi, Li, Yi: "Effects of shRNA targeting maspin on invasion of gastric carcinoma SGC7901 cell line." in: Oncology reports, Vol. 25, Issue 1, pp. 259-65, (2010) (PubMed).

Target Details for SERPINB5

Target: SERPINB5 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 5 (SERPINB5))

Alternative Name: SERPINB5 (SERPINB5 Products)

Synonyms: 1110036M19Rik antibody, AI462524 antibody, AI646751 antibody, Maspin antibody, PI-5 antibody, Spi7 antibody, ovalbumin antibody, PI5 antibody, maspin antibody, Pi5 antibody, serine (or cysteine) peptidase inhibitor, clade B, member 5 antibody, serpin family B member 5 antibody, serpin peptidase inhibitor, clade B (ovalbumin), member 5 S homeolog antibody, Serpinb5 antibody, SERPINB5 antibody, serpinb5.S antibody

Background: SERPINB5 is also known as PI5 or maspin. Maspin (mammary serine protease inhibitor) is a protein that in humans is encoded by the SERPINB5 gene. Maspin is expressed in the skin, prostate, testis, intestine, tongue, lung, and the thymus. Maspin is a member of the serpin superfamily of serine protease inhibitors.[1] The primary function of most members of this family is to regulate the breakdown of proteins by inhibiting the catalytic activity of proteinases. Through this mechanism of action, serpins regulate a number of cellular processes includingphagocytosis, coagulation, and fibrinolysis.

Synonyms: mammary serine proteinase inhibitor antibody|Maspin antibody|Ovalbumin antibody|Peptidase inhibitor 5 antibody|PI-5 antibody|PI5 antibody|Protease inhibitor 5 antibody|Serine (or cysteine) peptidase inhibitor, clade B, member 5 antibody|Serpin B5 antibody|serpin peptidase inhibitor antibody|Serpin peptidase inhibitor, clade B (ovalbumin), member 5 antibody|SERPINB5 antibody|SPB5_HUMAN antibody|Spi5 antibody|Spi7 antibody

Gene ID: 5268

UniProt: P36952

Pathways: p53 Signaling