EYA1 antibody (Internal Region)

Catalog No. ABIN487975

This Goat Polyclonal antibody specifically detects EYA1 in WB and EIA. It exhibits reactivity toward Human, Mouse, Rat, Cow and Dog.

Quick Overview for EYA1 antibody (Internal Region) (ABIN487975)

Target: EYA1 (Eyes Absent Homolog 1 (EYA1))

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This EYA1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-EYA1 Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to EYA1.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine, Bovine.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Immunogen: Peptide with sequence C-TDPTAEYSTIHSP, from the internal region of the protein sequence according to NP_742057.1, NP_000494.2, NP_742056.1.

Application Details

Application Notes: Peptide ELISA: 1/16000. Western blot: Approx 55 kDa band observed in lysates of cell lline HEK293 (calculated MWof 60.5 kDa according to NP_742056.1). Recommended concentration: 1 - 3 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for EYA1

Target: EYA1 (Eyes Absent Homolog 1 (EYA1))

Alternative Name: EYA1

Background: EYA1 (Eyes Absent Homolog 1) is a member of the eyes absent (EYA) family of proteins. EYA1 plays a role in transcriptional regulation during organogenesis. It is thought play a role in the development of the kidney, branchial arches, eye and ear. EYA1 is thought to coactivate SIX2, SIX4 and SIX5. EYA1 has intrinsic protein phosphatase activity, and dephosphorylates proteins such as Histone H2.AX phosphorylated on Y142. It is thought to play a role in promoting efficient DNA repair over apoptosis in response to genotypic stress.Synonyms: Eyes absent homolog 1

Gene ID: 2138

NCBI Accession: NP_000494

UniProt: Q99502

Pathways: Sensory Perception of Sound, Positive Regulation of Response to DNA Damage Stimulus