CCAR1 antibody (Internal Region)

Catalog No. ABIN487983

The Goat Polyclonal anti-CCAR1 antibody (ABIN487983) specifically detects CCAR1 in WB and EIA. The antibody is reactive with Human, Mouse, Rat, Dog, Cow and Pig samples.

Quick Overview for CCAR1 antibody (Internal Region) (ABIN487983)

Target: CCAR1 (Cell Division Cycle and Apoptosis Regulator 1 (CCAR1))

Reactivity: Human, Mouse, Rat, Dog, Cow, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This CCAR1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CCAR1 Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts to CCAR1.

Cross-Reactivity (Details): Species reactivity (expected):Canine, Bovine, Porcine.
Species reactivity (tested):Mouse, Rat.

Purification: Affinity chromatography

Immunogen: Peptide with sequence RYCKERPSKDKE, from the internal region (near C-Terminus) of the protein sequence according to NP_060707.2.

Application Details

Application Notes: Peptide ELISA: 1/16000. Western blot: Approx 120 kDa band observed in Mouse and Rat Skeletal Muscle lysates(calculated MW of 130 kDa according to Mouse NP_080477.1 and Rat NP_001102005.1). Recommended concentration: 1 - 3 μg/mL. An additional band of unknown identity was also consistently observed at 30 kDa. Thisband was successfully blocked by incubation with the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for CCAR1

Target: CCAR1 (Cell Division Cycle and Apoptosis Regulator 1 (CCAR1))

Alternative Name: CCAR1

Background: DIS is thought to be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Therefore DIS would have a role in cell cycle progression and/or cell proliferation.Synonyms: CARP1, Cell cycle and apoptosis regulatory protein 1, Cell division cycle and apoptosis regulator protein 1, DIS, Death inducer with SAP domain

Gene ID: 55749

NCBI Accession: NP_060707

UniProt: Q8IX12