UNC45B antibody (Internal Region)

Catalog No. ABIN488030

The Goat Polyclonal anti-UNC45B antibody is suitable to detect UNC45B in samples from Human, Mouse, Rat and Dog. It has been validated for WB and EIA.

Quick Overview for UNC45B antibody (Internal Region) (ABIN488030)

Target: UNC45B (Unc-45 Homolog B (UNC45B))

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This UNC45B antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-UNC45B Antibody

Binding Specificity: Internal Region

Specificity: This antibody is expected to recognise both reported isoforms (NP_775259.1 and NP_001028748.1).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat and Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RSDKLRQKIFKER, from the internal region of the protein sequence according to NP_775259.1, NP_001028748.1.

Application Details

Application Notes: Peptide ELISA: Detection Limit Dilution: 1/2000. Western blot: 0.3-1 μg/mL. Approx 90 kDa band observed in Human Skeletal Muscle lysates(calculated MW of 100 kDa according to NP_775259.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C.

Target Details for UNC45B

Target: UNC45B (Unc-45 Homolog B (UNC45B))

Alternative Name: UNC45B

Background: UNC45B may play a role in sarcomere formation during muscle cell development. It may act as co-chaperone for HSP90 and may be necessary for proper folding of myosin. There are three named isoforms.Synonyms: CMYA4, Protein unc-45 homolog B, SMUNC45, UNC-45B, UNC45

Gene ID: 146862

NCBI Accession: NP_001028748

UniProt: Q8IWX7