IL3RA antibody (PE)

Catalog No. ABIN488142

This anti-IL3RA antibody is a Mouse Monoclonal antibody detecting IL3RA in FACS. Suitable for Human.

Quick Overview for IL3RA antibody (PE) (ABIN488142)

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL3RA antibody is conjugated to PE

Application: Flow Cytometry (FACS)

Clone: 6H6

Product Details anti-IL3RA Antibody (PE)

Specificity: This antibody reacts with Human CD123 antigen (IL-3 receptor alpha-chain).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Protein-A Sepharose Chromatography.

Immunogen: COS cells overexpressing IL-3 receptor alpha-chain. Remarks: Hybridoma was established by fusion of mouse myeloma cell NS-1 with Balb/c mouse splenocyte

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry: 20 μL (Ready for Use). Positive Control: Transfectant cells. Detailed procedure is provided in the following Protocols.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Flow Cytometric Aanalysis for Adherent CellsProtocol 1We usually use Fisher tubes or equivalents as reaction tubes for all steps after 2). 1) Detach the cells from culture dish by trypsinization. *Excessive trypsinization mayreduce the antigenicity. 2) Wash the cells 3 times with washing buffer [PBS containing 2% fetal calf serum (FCS)and 0. 1% NaN3]. 3) Resuspend the cells with washing buffer (5x10e6 cells/mL). 4) Add 50 µL of the cell suspension into each tube, and centrifuge at 500 x g for 1 minute atroom temperature (20~25°C). Remove supernatant by careful aspiration. 5) Add 10 µL of normal goat serum containing 1 mg/mL normal human IgG and 0. 1% NaN3to the cell pellet after tapping. Mix well and incubate for 5 minutes at room temperature(20~25°C)6) Add 20 µL of the PE labeled CD123 monoclonal antibody (6H6). Mix well, and incubatefor 30 minutes at room temperature. 7) Add 1 mL of the washing buffer followed by centrifugation at 500 x g for 1 minute at roomtemperature (20~25°C). Remove supernatant by careful aspiration. 8) Resuspend the cells with 500 µL of the washing buffer and analyze by a flow cytometer. Positive Control for Flow Cytometry: transfectant

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 1 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

Target Details for IL3RA

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Alternative Name: CD123 / IL3RA

Background: CD123 is the IL-3-specific component of the IL-3 receptor. CD123 binds IL-3 only in combination with CDw131, the Synonyms: IL-3R-alpha, IL3 receptor alpha, Interleukin-3 receptor subunit alpha

Gene ID: 3563

NCBI Accession: NP_002174

UniProt: P26951

Pathways: JAK-STAT Signaling