ADH4 antibody (AA 218-380)

Catalog No. ABIN4886428

This Rabbit Polyclonal antibody specifically detects ADH4 in WB. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ADH4 antibody (AA 218-380) (ABIN4886428)

Target: ADH4 (Alcohol Dehydrogenase 4 (Class II), pi Polypeptide (ADH4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADH4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ADH4 Antibody

Binding Specificity: AA 218-380

Purpose: Anti-ADH4 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ADH4 Antibody Picoband® (ABIN4886428). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human ADH4 recombinant protein (Position: K218-F380). Human ADH4 shares 68.7% and 71.8% amino acid (aa) sequence identity with mouse and rat ADH4, respectively.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Li M, Edenberg HJ (1998). "Function of cis-acting elements in human alcohol dehydrogenase 4 (ADH4) promoter and role of C/EBP proteins in gene expression.". DNA Cell Biol. 17 (4): 387-97. 2. Luo X, Kranzler HR, Zuo L, et al. (2006). "ADH4 gene variation is associated with alcohol and drug dependence: results from family controlled and population-structured association studies.".Pharmacogenet. Genomics 15(11): 755-68.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ADH4

Target: ADH4 (Alcohol Dehydrogenase 4 (Class II), pi Polypeptide (ADH4))

Alternative Name: ADH4

Background:

Synonyms: Alcohol dehydrogenase 4,1.1.1.1,Alcohol dehydrogenase class II pi chain,ADH4,

Tissue Specificity: Plasma. Expressed primarily in liver. .

Background: Alcohol dehydrogenase 4 is an enzyme that in humans is encoded by the ADH4 gene. This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.

Molecular Weight: 40 kDa

Gene ID: 127

UniProt: P08319

Pathways: Transition Metal Ion Homeostasis