ADH4 antibody (Middle Region)

Catalog No. ABIN2781328

There is 1 publication for this product available. The Rabbit Polyclonal anti-ADH4 antibody is suitable to detect ADH4 in samples from Human, Rat, Cow, Pig, Dog and Rabbit. It has been validated for WB and IHC.

Quick Overview for ADH4 antibody (Middle Region) (ABIN2781328)

Target: ADH4 (Alcohol Dehydrogenase 4 (Class II), pi Polypeptide (ADH4))

Reactivity: Human, Rat, Cow, Pig, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADH4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-ADH4 Antibody

Binding Specificity: Middle Region

Purpose: ADH4 Antibody - middle region

Sequence: NSEKFVKAKA LGATDCLNPR DLHKPIQEVI IELTKGGVDF ALDCAGGSET

Predicted Reactivity: Cow: 86%, Dog: 86%, Human: 100%, Pig: 86%, Rabbit: 79%, Rat: 79%

Characteristics: This is a rabbit polyclonal antibody against ADH4. It was validated on Western Blot and immunohistochemistry.

Purification: Protein A purified

Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human ADH4

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8094349

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ADH4 antibody (ABIN2781328)

Hu, Lausted, Yoo, Yan, Brightman, Chen, Wang, Bu, Hood: "Quantitative liver-specific protein fingerprint in blood: a signature for hepatotoxicity." in: Theranostics, Vol. 4, Issue 2, pp. 215-28, (2014) (PubMed).

Target Details for ADH4

Target: ADH4 (Alcohol Dehydrogenase 4 (Class II), pi Polypeptide (ADH4))

Alternative Name: ADH4

Background:

Background Information: ADH4, class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole.This gene encodes class II alcohol dehydrogenase 4 pi subunit, which is a member of the alcohol dehydrogenase family. Members of this enzyme family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. Class II alcohol dehydrogenase is a homodimer composed of 2 pi subunits. It exhibits a high activity for oxidation of long-chain aliphatic alcohols and aromatic alcohols and is less sensitive to pyrazole. This gene is localized to chromosome 4 in the cluster of alcohol dehydrogenase genes.

Gene Name: Alcohol dehydrogenase 4 (class II), pi polypeptide

Alternative Symbols: ADH-2, HEL-S-4

Protein Name: Alcohol dehydrogenase 4

Molecular Weight: 42kDa

Gene ID: 127

NCBI Accession: NP_000661

UniProt: P08319

Pathways: Transition Metal Ion Homeostasis