ADO antibody (AA 49-261)

Catalog No. ABIN4886431

This Rabbit Polyclonal antibody specifically detects ADO in WB, IHC, FACS, ICC and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ADO antibody (AA 49-261) (ABIN4886431)

Target: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ADO antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF)

Product Details anti-ADO Antibody

Binding Specificity: AA 49-261

Purpose: Anti-ADO Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-ADO Antibody Picoband® (ABIN4886431). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human ADO recombinant protein (Position: E49-E261). Human ADO shares 90.1% amino acid (aa) sequence identity with mouse ADO.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Dominy, J. E., Jr., Simmons, C. R., Hirschberger, L. L., Hwang, J., Coloso, R. M., Stipanuk, M. H. Discovery and characterization of a second mammalian thiol dioxygenase, cysteamine dioxygenase.J. Biol. Chem. 282: 25189-25198, 2007.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for ADO

Target: ADO (2-Aminoethanethiol (Cysteamine) Dioxygenase (ADO))

Alternative Name: ADO

Background:

Synonyms: 2-aminoethanethiol dioxygenase,1.13.11.19,Cysteamine dioxygenase,ADO,C10orf22,

Tissue Specificity: Widely expressed. Highest level in placenta, liver, muscle and kidney.

Background: Human thiol dioxygenases include cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). CDO adds 2 oxygen atoms to free cysteine, whereas ADO adds 2 oxygen atoms to free cysteamine to form hypotaurine. It is demonstrated that mouse Ado has strong and specific dioxygenase activity in vitro towards cysteamine but not cysteine. Recombinant Ado was shown to bind iron. Overexpression of Ado in HepG2/C3A cells increased the production of hypotaurine from cysteamine. Similar results were found with human ADO. When endogenous expression of ADO was reduced by RNA-mediated interference, hypotaurine production decreased. It is also noted that the demonstration of high levels of ADO in brain challenges the previous assumption that most of the taurine in the brain is a consequence of CDO activity.

Molecular Weight: 28 kDa, 30 kDa