AFF4 antibody (N-Term)

Catalog No. ABIN4886436

The Rabbit Polyclonal anti-AFF4 antibody (ABIN4886436) specifically detects AFF4 in WB, FACS, IP and IHC. The antibody is reactive with Human samples.

Quick Overview for AFF4 antibody (N-Term) (ABIN4886436)

Target: AFF4 (AF4/FMR2 Family, Member 4 (AFF4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFF4 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Product Details anti-AFF4 Antibody

Binding Specificity: N-Term

Purpose: Anti-AFF4 Antibody

Sequence: RNVLRMKERE RRNQEIQQGE DAFPPSSPLF AEPYKVTSKE DKLSSRIQ

Specificity: No cross reactivity with other proteins.

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Predicted Reactivity: identical to the related mouse sequence.

Characteristics: Anti-AFF4 Antibody. Tested in Flow Cytometry, IP, IHC, WB applications. This antibody reacts with Human.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human AFF4, identical to the related mouse sequence.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Mouse, Rat Immunoprecipitation, 0.5-2 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for AFF4

Target: AFF4 (AF4/FMR2 Family, Member 4 (AFF4))

Alternative Name: AFF4

Background:

Background: The AFF4 gene encodes a scaffold protein that functions as a core component of the super elongation complex (SEC), which is involved in transcriptional regulation during embryogenesis. The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. This gene is mapped to chromosome 5q31.

Gene Full Name: ALF transcription elongation factor 4

Molecular Weight: 150 kDa

Gene ID: 27125

UniProt: Q9UHB7