AFG3L2 antibody (AA 168-250)

Catalog No. ABIN4886437

This Rabbit Polyclonal antibody specifically detects AFG3L2 in WB, IP, IF, ICC and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for AFG3L2 antibody (AA 168-250) (ABIN4886437)

Target: AFG3L2 (AFG3-Like Protein 2 (AFG3L2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AFG3L2 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Product Details anti-AFG3L2 Antibody

Binding Specificity: AA 168-250

Purpose: Anti-AFG3L2 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-AFG3L2 Antibody (ABIN4886437). Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human AFG3L2 recombinant protein (Position: R168-D250). Human AFG3L2 shares 100% amino acid (aa) sequence identity with mouse AFG3L2.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for AFG3L2

Target: AFG3L2 (AFG3-Like Protein 2 (AFG3L2))

Alternative Name: AFG3L2

Background:

Synonyms: AFG3-like protein 2,3.4.24.-,Paraplegin-like protein,AFG3L2,

Tissue Specificity: Ubiquitous. Highly expressed in the cerebellar Purkinje cells. .

Background: AFG3L2 is the catalytic subunit of the m-AAA protease, an ATP-dependent proteolytic complex of the mitochondrial inner membrane that degrades misfolded proteins and regulates ribosome assembly. In humans, it is encoded by the AFG3L2 gene. This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. And this gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders as well as spastic ataxia-neuropathy syndrome.

Molecular Weight: 89 kDa

Gene ID: 10939

UniProt: Q9Y4W6

Pathways: Skeletal Muscle Fiber Development