Adenylate Kinase 1 antibody (C-Term)

Catalog No. ABIN4886445

This anti-Adenylate Kinase 1 antibody is a Rabbit Polyclonal antibody detecting Adenylate Kinase 1 in WB, IHC and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for Adenylate Kinase 1 antibody (C-Term) (ABIN4886445)

Target: Adenylate Kinase 1 (AK1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Adenylate Kinase 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-Adenylate Kinase 1 Antibody

Binding Specificity: AA 149-189, C-Term

Purpose: Anti-Adenylate Kinase 1/AK1 Antibody Picoband®

Sequence: RLETYYKATE PVIAFYEKRG IVRKVNAEGS VDSVF

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Adenylate Kinase 1/AK1 Antibody Picoband® (ABIN4886445). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Adenylate Kinase 1, different from the related mouse sequence by seven amino acids, and from the related rat sequence by four amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Bianchi, P., Zappa, M., Bredi, E., Vercellati, C., Pelissero, G., Barraco, F., Zanella, A. A case of complete adenylate kinase deficiency due to a nonsense mutation in AK-1 gene (arg107-to-stop, CGA-to-TGA) associated with chronic haemolytic anaemia. Brit. J. Haemat. 105: 75-79, 1999. 2. Qualtieri, A., Pedace, V., Bisconte, M. G., Bria, M., Gulino, B., Andreoli, V., Brancati, C. Severe erythrocyte adenylate kinase deficiency due to homozygous A-to-G substitution at codon 164 of human AK1 gene associated with chronic haemolytic anaemia. Brit. J. Haemat. 99: 770-776, 1997.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Adenylate Kinase 1

Target: Adenylate Kinase 1 (AK1)

Alternative Name: AK1

Background:

Synonyms: Adenylate kinase isoenzyme 1 ,AK 1 ,2.7.4.3 ,2.7.4.6 ,ATP-AMP transphosphorylase 1 ,ATP:AMP phosphotransferase ,Adenylate monophosphate kinase ,Myokinase ,AK1 ,

Tissue Specificity: Widely expressed. Highest level in placenta, liver, muscle and kidney.

Background: This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Molecular Weight: 22 kDa

Gene ID: 203

UniProt: P00568

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process