Cyclin T1 antibody (Middle Region)

Catalog No. ABIN4886509

This Rabbit Polyclonal antibody specifically detects Cyclin T1 in WB, IHC, FACS, IF, IHC (fro) and ICC. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Cyclin T1 antibody (Middle Region) (ABIN4886509)

Target: Cyclin T1 (CCNT1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin T1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunocytochemistry (ICC)

Product Details anti-Cyclin T1 Antibody

Binding Specificity: AA 375-410, Middle Region

Purpose: Anti-Cyclin T1/CCNT1 Antibody Picoband®

Sequence: QKQNSKSVPS AKVSLKEYRA KHAEELAAQK RQLENM

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-Cyclin T1/CCNT1 Antibody Picoband® (ABIN4886509). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Cyclin T1, different from the related mouse sequence by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. "Entrez Gene: CCNT1 cyclin T1". 2. Peng J, Zhu Y, Milton JT, Price DH (April 1998). "Identification of multiple cyclin subunits of human P-TEFb". Genes Dev. 12 (5): 755-62. 3. Wei P, Garber ME, Fang SM, Fischer WH, Jones KA (March 1998). "A novel CDK9-associated C-type cyclin interacts ly with HIV-1 Tat and mediates its high-affinity, loop-specific binding to TAR RNA". Cell92 (4): 451-62.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for Cyclin T1

Target: Cyclin T1 (CCNT1)

Alternative Name: CCNT1

Background:

Synonyms: Cyclin-T1,CycT1,Cyclin-T,CCNT1,

Tissue Specificity: Ubiquitously expressed.

Background: Cyclin-T1 is a protein that in humans is encoded by the CCNT1 gene. This gene encodes a member of the highly conserved cyclin C subfamily. The encoded protein tightly associates with cyclin-dependent kinase 9, and is a major subunit of positive transcription elongation factor b (p-TEFb). In humans, there are multiple forms of positive transcription elongation factor b, which may include one of several different cyclins along with cyclin-dependent kinase 9. The complex containing the encoded cyclin and cyclin-dependent kinase 9 acts as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and is both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner are also involved in triggering transcript elongation through phosphorylation of the carboxy-terminal domain of the largest RNA polymerase II subunit. Overexpression of this gene is implicated in tumor growth. Alternative splicing results in multiple transcript variants.

Molecular Weight: 81 kDa

Gene ID: 904

UniProt: O60563