CCT4 antibody (AA 452-539)

Catalog No. ABIN4886514

The Rabbit Polyclonal anti-CCT4 antibody (ABIN4886514) specifically detects CCT4 in WB, IHC, IF, IP, FACS, ICC and IHC (fro). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CCT4 antibody (AA 452-539) (ABIN4886514)

Target: CCT4 (Chaperonin Containing TCP1, Subunit 4 (Delta) (CCT4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCT4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-CCT4 Antibody

Binding Specificity: AA 452-539

Purpose: Anti-TCP1 delta/CCT4 Antibody

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-TCP1 delta/CCT4 Antibody (ABIN4886514). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human CCT4 recombinant protein (Position: R452-R539). Human CCT4 shares 97.7% amino acid (aa) sequence identity with both mouse and rat CCT4.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human, Mouse, Rat
Immunoprecipitation, 0.5-2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for CCT4

Target: CCT4 (Chaperonin Containing TCP1, Subunit 4 (Delta) (CCT4))

Alternative Name: CCT4

Background:

Synonyms: T-complex protein 1 subunit delta,TCP-1-delta,CCT-delta,Stimulator of TAR RNA-binding,CCT4,CCTD, SRB,

Tissue Specificity: Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta. .

Background: T-complex protein 1 subunit delta is a protein that in humans is encoded by the CCT4 gene. This gene is mapped to 2p15. The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha-transducin.

Molecular Weight: 58 kDa

Gene ID: 10575

UniProt: P50991

Pathways: Embryonic Body Morphogenesis