CD27 antibody (AA 20-191)

Catalog No. ABIN4886519

Product Details anti-CD27 Antibody

Target: CD27 (CD27 Molecule (CD27))

Binding Specificity: AA 20-191

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD27 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for CD27 antigen(CD27) detection. Tested with WB, IHC-P, FCM in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for CD27 antigen(CD27) detection. Tested with WB, IHC-P, FCM in Human.
Gene Name: CD27 Molecule
Protein Name: CD27 antigen

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human CD27 recombinant protein (Position: A20-R191). Human CD27 shares 62.8% amino acid (aa) sequence identity with mouse CD27.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Flow Cytometry: Concentration:1-3 μg/1x106 cells, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27 (CD27 Products)

Synonyms: TNFRSF7 antibody, S152 antibody, Tnfrsf7 antibody, Tp55 antibody, S152. LPFS2 antibody, T14 antibody, CD27 molecule antibody, CD27 antigen antibody, CD27 antibody, Cd27 antibody

Background: CD27 is a member of the tumor necrosis factor receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. And this receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

Synonyms: CD 27 | CD27 | CD27 antigen | CD27 Molecule | CD27L receptor | LPFS2 | S152 | T14 | TNFRSF 7 | TNFRSF7 | TNFSF7 | Tp 55 | Tp55 | P26842

Gene ID: 939

UniProt: P26842

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints