CD44 antibody (C-Term)

Catalog No. ABIN4886529

This anti-CD44 antibody is a Rabbit Polyclonal antibody detecting CD44 in FACS, WB and IHC. Suitable for Human and Rat. This Primary Antibody has been cited in 26+ publications.

Quick Overview for CD44 antibody (C-Term) (ABIN4886529)

Target: CD44

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD44 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC)

Product Details anti-CD44 Antibody

Binding Specificity: AA 728-742, C-Term

Purpose: Anti-CD44 antigen CD44 Antibody Picoband®

Sequence: DETRNLQNVD MKIGV

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-CD44 antigen CD44 Antibody (ABIN4886529). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CD44, different from the related mouse and rat sequences by one amino acid.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. Screaton GR, Bell MV, Jackson DG, Cornelis FB, Gerth U, Bell JI. Genomic structure of DNA encoding the lymphocyte homing receptor CD44 reveals at least 12 alternatively spliced exons. Proc Natl Acad Sci U S A. 1992 Dec 15, 89(24):12160-4. 2. Telen MJ, Udani M, Washington MK, Levesque MC, Lloyd E, Rao N. A blood group-related polymorphism of CD44 abolishes a hyaluronan-binding consensus sequence without preventing hyaluronan binding. J Biol Chem. 1996 Mar 22, 271(12):7147-53. 3. Weber GF, Ashkar S, Glimcher MJ, Cantor H. Receptor-ligand interaction between CD44 and osteopontin (Eta-1). Science. 1996 Jan 26, 271(5248):509-12. 4. Sherman L, Wainwright D, Ponta H, Herrlich P. A splice variant of CD44 expressed in the apical ectodermal ridge presents fibroblast growth factors to limb mesenchyme and is required for limb outgrowth. Genes Dev. 1998 Apr 1, 12(7):1058-71. 5. van Royen N, Voskuil M, Hoefer I, Jost M, de Graaf S, Hedwig F, Andert JP, Wormhoudt TA, Hua J, Hartmann S, Bode C, Buschmann I, Schaper W, van der Neut R, Piek JJ, Pals ST.CD44 regulates arteriogenesis in mice and is differentially expressed in patients with poor and good collateralization. Circulation. 2004 Apr 6, 109(13):1647-52.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-CD44 antibody (ABIN4886529)

Zhang, Yan, Liu, Li, Zhang, Pang, Hu, Zhao, Xie, Zhou, Wang, Xie: "Smad3-related miRNAs regulated oncogenic TRIB2 promoter activity to effectively suppress lung adenocarcinoma growth." in: Cell death & disease, Vol. 7, Issue 12, pp. e2528, (2017) (PubMed).

Ding, Chang, Niu, Dai, Geng, Li, Guo, Xu: "Overexpression of transcription factor Foxa2 and Hnf1α induced rat bone mesenchymal stem cells into hepatocytes." in: Cytotechnology, Vol. 68, Issue 5, pp. 2037-47, (2016) (PubMed).

Zhang, Shang, Hao, Zheng, Li, Liang, Cui, Liu: "Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats." in: American journal of translational research, Vol. 7, Issue 11, pp. 2176-86, (2016) (PubMed).

Zhang, Wang, Dai, Cai, Zhan, Gang, Hu, Xia, Zhang: "Lentivirus-mediated PLCγ1 gene short-hairpin RNA suppresses tumor growth and metastasis of human gastric adenocarcinoma." in: Oncotarget, Vol. 7, Issue 7, pp. 8043-54, (2016) (PubMed).

Yin, Zhou, Wang, Ma, Li, Wang, She, Li: "CD44 promotes the migration of bone marrow-derived mesenchymal stem cells toward glioma." in: Oncology letters, Vol. 11, Issue 4, pp. 2353-2358, (2016) (PubMed).

Dai, Chen, Ding, Niu, Fan, Xu: "Induction of Functional Hepatocyte-Like Cells by Overexpression of FOXA3 and HNF4? in Rat Bone Marrow Mesenchymal Stem Cells." in: Cells, tissues, organs, Vol. 200, Issue 2, pp. 132-40, (2015) (PubMed).

Yuan, Liu, Li, Li, Sun, Xu, Man, Fu: "Effects of BMSCs interactions with adventitial fibroblasts in transdifferentiation and ultrastructure processes." in: International journal of clinical and experimental pathology, Vol. 7, Issue 7, pp. 3957-65, (2015) (PubMed).

Li, Huang, Zheng, Liu, Niu, Huang, Zhao, Fan: "Effect of substrate stiffness on the functions of rat bone marrow and adipose tissue derived mesenchymal stem cells in vitro." in: Journal of biomedical materials research. Part A, Vol. 102, Issue 4, pp. 1092-101, (2014) (PubMed).

Li, Liu, Mei, Chen, Liu, Zhao: "Overexpression of inducible nitric oxide synthase impairs the survival of bone marrow stem cells transplanted into rat infarcted myocardium." in: Life sciences, Vol. 106, Issue 1-2, pp. 50-7, (2014) (PubMed).

Du, Liu, Dang, Zhu, Su, Fan, Tan, Wang, Fang: "Comparison of administration routes for adipose-derived stem cells in the treatment of middle cerebral artery occlusion in rats." in: Acta histochemica, Vol. 116, Issue 6, pp. 1075-84, (2014) (PubMed).

Shi, Han, Deng, Zhang, Shen, Gong, Zhang, Sun: "Dual drugs (microRNA-34a and paclitaxel)-loaded functional solid lipid nanoparticles for synergistic cancer cell suppression." in: Journal of controlled release : official journal of the Controlled Release Society, Vol. 194, pp. 228-37, (2014) (PubMed).

Hefei, Yu, Haiqing, Xiao, Jingyuan, Dongjun: "Morphological Characteristics and Identification of Islet-like Cells Derived From Rat Adipose-derived Stem Cells Cocultured with Pancreas Adult Stem Cells." in: Cell biology international, (2014) (PubMed).

Fu, Lv, Zhuang, Chen, Lv, Li, Wang: "An effective inducer of dopaminergic neuron-like differentiation." in: Neural regeneration research, Vol. 8, Issue 5, pp. 427-34, (2014) (PubMed).

Lin, Li, Han, Li, Lu, Bai, Chen: "Folium Sennae protects against hydroxyl radical-induced DNA damage via antioxidant mechanism: an in vitro study." in: Botanical studies, Vol. 55, Issue 1, pp. 16, (2014) (PubMed).

Dai, Li, Zhou, Chen, Chen, Xiao: "Genistein promotion of osteogenic differentiation through BMP2/SMAD5/RUNX2 signaling." in: International journal of biological sciences, Vol. 9, Issue 10, pp. 1089-98, (2013) (PubMed).

Huang, Yuan, Yang: "Effects of total flavonoids from Drynaria fortunei on the proliferation and osteogenic differentiation of rat dental pulp stem cells." in: Molecular medicine reports, Vol. 6, Issue 3, pp. 547-52, (2012) (PubMed).

Zhang, Lan, Yen, Chen, Pei, Qin, Zhang: "Therapeutic gene expression in transduced mesenchymal stem cells can be monitored using a reporter gene." in: Nuclear medicine and biology, Vol. 39, Issue 8, pp. 1243-50, (2012) (PubMed).

Hou, Zou, Ge, Shen, Wang: "The critical role of CD133(+)CD44(+/high) tumor cells in hematogenous metastasis of liver cancers." in: Cell research, Vol. 22, Issue 1, pp. 259-72, (2012) (PubMed).

Han, Liu, Wang, Mo, Bi, Liu, Fan, Chen, Wu: "Re-expression of miR-21 contributes to migration and invasion by inducing epithelial-mesenchymal transition consistent with cancer stem cell characteristics in MCF-7 cells." in: Molecular and cellular biochemistry, Vol. 363, Issue 1-2, pp. 427-36, (2012) (PubMed).

Han, Liu, Wang, Chen, Xu, Sun, Zhao, Qu, Fan, Wu: "Antagonism of miR-21 reverses epithelial-mesenchymal transition and cancer stem cell phenotype through AKT/ERK1/2 inactivation by targeting PTEN." in: PLoS ONE, Vol. 7, Issue 6, pp. e39520, (2012) (PubMed).

Target Details for CD44

Target: CD44

Alternative Name: CD44

Background:

Synonyms: CD44 antigen,CDw44,Epican,Extracellular matrix receptor III,ECMR-III,GP90 lymphocyte homing/adhesion receptor,HUTCH-I,Heparan sulfate proteoglycan,Hermes antigen,Hyaluronate receptor,Phagocytic glycoprotein 1,PGP-1,Phagocytic glycoprotein I,PGP-I,CD44,CD44,LHR, MDU2, MDU3, MIC4,

Tissue Specificity: Isoform 10 (epithelial isoform) is expressed by cells of epithelium and highly expressed by carcinomas. Expression is repressed in neuroblastoma cells.

Background: The CD44 gene, which is a transmembrane protein, is expressed as a family of molecular isoforms generated from alternative RNA splicing and posttranslational modifications. The gene, which contains 19 exons spanning some 50 kb of genomic DNA, is a widely expressed integral membrane protein that acts as a receptor for hyaluronan (HA) and is important to cell-extracellular matrix interaction. CD44 binding with HA can play an important role in cellular aggregation and tumor cell growth. CD44 is necessary for limb development and functions in a novel growth factor presentation mechanism. A specific CD44 splice variant is crucial for the proliferation of these mesenchymal cells. CD44 glycoproteins are involved in leukocyte extravasation but also in the regulation of growth factor activation, stability, and signaling. Moreover, it plays a pivotal role in arteriogenesis.

Sequence Similarities: Contains 1 Link domain.

Molecular Weight: 82 kDa

UniProt: P16070

Pathways: Glycosaminoglycan Metabolic Process, Autophagy, Negative Regulation of intrinsic apoptotic Signaling