CD58 antibody (AA 29-215)

Catalog No. ABIN4886530

Product Details anti-CD58 Antibody

Target: CD58

Binding Specificity: AA 29-215

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD58 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Purpose: Rabbit IgG polyclonal antibody for Lymphocyte function-associated antigen 3(CD58) detection. Tested with WB, IHC-P, FCM in Human.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Lymphocyte function-associated antigen 3(CD58) detection. Tested with WB, IHC-P, FCM in Human.
Gene Name: CD58 Molecule
Protein Name: Lymphocyte function-associated antigen 3

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human LFA3 recombinant protein (Position: F29-R215).

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Flow Cytometry: Concentration:1-3 μg/1x106 cells, Tested Species: Human
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for CD58

Target: CD58

Alternative Name: CD58 (CD58 Products)

Synonyms: LFA-3 antibody, LFA3 antibody, ag3 antibody, CD58 molecule antibody, CD58 antibody

Background: CD58, or lymphocyte function-associated antigen 3 (LFA-3), is a cell adhesion molecule expressed on Antigen Presenting Cells (APC), particularly macrophages. It binds to CD2 (LFA-2) on T cells and is important in strengthening the adhesion between the T cells and Professional Antigen Presenting Cells. This adhesion occurs as part of the transitory initial encounters between T cells and Antigen Presenting Cells before T cell activation, when T cells are roaming the lymph nodes looking at the surface of APCs for peptide:MHC complexes the T-cell receptors are reactive to. The LFA3 gene is mapped to chromosome 1p13, which is the same location as CD2.

Synonyms: AG3 | CD58 | CD58 antigen | CD58 Molecule | LFA 3 | LFA-3 | LFA3 | P19256

Gene ID: 965

UniProt: P19256