EPHX2 antibody (C-Term)

Catalog No. ABIN4886572

This Rabbit Polyclonal antibody specifically detects EPHX2 in WB and FACS. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for EPHX2 antibody (C-Term) (ABIN4886572)

Target: EPHX2 (Epoxide Hydrolase 2, Cytoplasmic (EPHX2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPHX2 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS)

Product Details anti-EPHX2 Antibody

Binding Specificity: AA 505-543, C-Term

Purpose: Anti-EPHX2 Antibody Picoband®

Sequence: QHMEDWIPHL KRGHIEDCGH WTQMDKPTEV NQILIKWLD

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-EPHX2 Antibody Picoband® (ABIN4886572). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human EPHX2, different from the related mouse sequence by seven amino acids, and from the related rat sequence by eight amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: Epoxide hydrolase 2, cytoplasmic". 2. Morisseau C, Hammock BD (2013). "Impact of soluble epoxide hydrolase and epoxyeicosanoids on human health".Annu. Rev. Pharmacol. Toxicol. 53: 37-58. 3. Lee CR, North KE, Bray MS, Fornage M, Seubert JM, Newman JW, Hammock BD, Couper DJ, Heiss G, Zeldin DC (May 2006). "Genetic variation in soluble epoxide hydrolase (EPHX2) and risk of coronary heart disease: The Atherosclerosis Risk in Communities (ARIC) study". Hum. Mol. Genet.15 (10): 1640-9.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for EPHX2

Target: EPHX2 (Epoxide Hydrolase 2, Cytoplasmic (EPHX2))

Alternative Name: EPHX2

Background:

Synonyms: Bifunctional epoxide hydrolase 2,Cytosolic epoxide hydrolase 2,CEH,3.3.2.10,Epoxide hydratase,Soluble epoxide hydrolase,SEH,Lipid-phosphate phosphatase,3.1.3.76,EPHX2,

Tissue Specificity: Ubiquitous. A high level expression is seen in secretory tissues.

Background: Soluble epoxide hydrolase (sEH) is a bifunctional enzyme that in humans is encoded by the EPHX2 gene. It is mapped to 8p21.2-p21.1. This gene encodes a member of the epoxide hydrolase family. The protein, found in both the cytosol and peroxisomes, binds to specific epoxides and converts them to the corresponding dihydrodiols. Mutations in this gene have been associated with familial hypercholesterolemia. Alternatively spliced transcript variants have been described.

Molecular Weight: 63 kDa

Gene ID: 2053

UniProt: P34913