FGF1 antibody (AA 16-155)

Catalog No. ABIN4886581

This Rabbit Polyclonal antibody specifically detects FGF1 in WB, IHC and ELISA. It exhibits reactivity toward Human, Mouse and Rat and has been mentioned in 2+ publications.

Quick Overview for FGF1 antibody (AA 16-155) (ABIN4886581)

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FGF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Product Details anti-FGF1 Antibody

Binding Specificity: AA 16-155

Purpose: Anti-FGF1 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-FGF1 Antibody Picoband® (ABIN4886581). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human FGF1 recombinant protein (Position: F16-D155). Human FGF1 shares 96.4% amino acid (aa) sequence identity with both mouse and rat FGF1.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/mL, -
Western blot, 0.1-0.5 μg/mL, Rat, Human
1. Burgess, W. H., Mehlman, T., Marshak, D. R., Fraser, B. A., Maciag, T. : Structural evidence that endothelial cell growth factor beta is the precursor of both endothelial cell growth factor alpha and acidic fibroblast growth factor. Proc. Nat. Acad. Sci. 83: 7216-7220, 1986. 2. Le Beau, M. M., Espinosa, R., III, Neuman, W. L., Stock, W., Roulston, D., Larson, R. A., Keinanen, M., Westbrook, C. A. : Cytogenetic and molecular delineation of the smallest commonly deleted region of chromosome 5 in malignant myeloid diseases. Proc. Nat. Acad. Sci. 90: 5484-5488, 1993. 3. Mergia, A., Eddy, R., Abraham, J. A., Fiddes, J. C., Shows, T. B. : The genes for basic and acidic fibroblast growth factors are on different human chromosomes. Biochem. Biophys. Res. Commun. 138: 644-651, 1986.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

References for anti-FGF1 antibody (ABIN4886581)

Zhang, Wang, Wang, Jiang, Yu, Chong, Fu: "Ferulic acid ameliorates nerve injury induced by cerebral ischemia in rats." in: Experimental and therapeutic medicine, Vol. 9, Issue 3, pp. 972-976, (2015) (PubMed).

Gu, Zhang, Wu, Xu, Cui, Yang, Zhao, Sun: "Differential expression of decorin, EGFR and cyclin D1 during mammary gland carcinogenesis in TA2 mice with spontaneous breast cancer." in: Journal of experimental & clinical cancer research : CR, Vol. 29, pp. 6, (2010) (PubMed).

Target Details for FGF1

Target: FGF1 (Fibroblast Growth Factor 1 (Acidic) (FGF1))

Alternative Name: FGF1

Background:

Synonyms: Fibroblast growth factor 1,FGF-1,Acidic fibroblast growth factor,aFGF,Endothelial cell growth factor,ECGF,Heparin-binding growth factor 1,HBGF-1,FGF1,FGFA,

Tissue Specificity: Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle. .

Background: Fibroblast growth factor 1 (acidic), also known as FGF1/ECGF/HBGF1, is a human gene which is mapped to 5q31. The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It also acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.

Molecular Weight: 24 kDa

Gene ID: 2246

UniProt: P05230

Pathways: RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway