AGO9 antibody

Catalog No. ABIN7540304

The Rabbit Polyclonal anti-AGO9 antibody is suitable to detect AGO9 in samples from Arabidopsis thaliana. It has been validated for IL, IP and WB.

Quick Overview for AGO9 antibody (ABIN7540304)

Target: AGO9 (Argonaute 9 (AGO9))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: Immunolocalization (IL), Immunoprecipitation (IP), Western Blotting (WB)

Product Details anti-AGO9 Antibody

Purpose: Anti-AGO9 | Argonaute 9

Cross-Reactivity (Details): Arabidopsis thaliana
Not reactive in: Populus sp.

Predicted Reactivity: Arabidopsis thaliana

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO9 protein sequence UniProt: Q84YI4

Application Details

Application Notes: 5 μg of antibody per 1 gram of a fresh tissue (IP), 1 : 10 000 (WB)

Comment:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for AGO9

Target: AGO9 (Argonaute 9 (AGO9))

Alternative Name: AGO9

Background: AGO9 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC).

Molecular Weight: 101 kDa

Gene ID: 832241

UniProt: Q84YI4