AGO9 antibody

Catalog No. ABIN7540304

The Rabbit Polyclonal anti-AGO9 antibody has been validated for IP and WB. It is suitable to detect AGO9 in samples from Arabidopsis thaliana.

Quick Overview for AGO9 antibody (ABIN7540304)

Target: AGO9 (Argonaute Family Protein (AGO9))

Reactivity: Arabidopsis thaliana

Host: Rabbit

Clonality: Polyclonal

Application: Immunoprecipitation (IP), Western Blotting (WB)

Product Details anti-AGO9 Antibody

Cross-Reactivity (Details): Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

Predicted Reactivity: Arabidopsis thaliana

Characteristics: Expected / apparent Molecular Weight of the Antigene: 101 kDa

Purification: serum

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana AGO9 protein sequence UniProt: Q84YI4, TAIR:At5g21150.

Application Details

Application Notes: 1: 10 000 (WB), 5 μg of antibody per 1 gram of a fresh tissue (IP)

Comment:

AGO expression may be tissue specific and using floral tissue is recommended where most of the AGOs are expressed the highest. Use of proteasome inhibitors as MG132 can help to stabilize AGO proteins during extraction procedure.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water.

Buffer: PBS pH 7.4

Handling Advice: Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for AGO9

Target: AGO9 (Argonaute Family Protein (AGO9))

Alternative Name: AGO9

Background: AGI Code: At5g21150
AGO9 belongs to a group of argonaute proteins which are catalytic component of the RNA-incudes silencing complex (RISC).

Molecular Weight: 101 kDa

UniProt: Q84YI4