HNRNPF antibody (N-Term)

Catalog No. ABIN4886622

This anti-HNRNPF antibody is a Rabbit Polyclonal antibody detecting HNRNPF in WB, IF, IHC, ICC, IHC (fro) and FACS. Suitable for Human, Mouse and Rat.

Quick Overview for HNRNPF antibody (N-Term) (ABIN4886622)

Target: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HNRNPF antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Product Details anti-HNRNPF Antibody

Binding Specificity: AA 23-53, N-Term

Purpose: Anti-HnRNP F/HNRNPF Antibody Picoband®

Sequence: SVEDVQNFLS DCTIHDGAAG VHFIYTREGR Q

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-HnRNP F/HNRNPF Antibody Picoband® (ABIN4886622). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HnRNP F, different from the related mouse and rat sequences by two amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. "Entrez Gene: HNRPF heterogeneous nuclear ribonucleoprotein F". 2. Honore B, Rasmussen HH, Vorum H, Dejgaard K, Liu X, Gromov P, Madsen P, Gesser B, Tommerup N, Celis JE (Jan 1996). "Heterogeneous nuclear ribonucleoproteins H, H', and F are members of a ubiquitously expressed subfamily of related but distinct proteins encoded by genes mapping to different chromosomes". J Biol Chem. 270 (48): 28780-9. 3. Matunis MJ, Xing J, Dreyfuss G (1994). "The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization.". Nucleic Acids Res. 22 (6): 1059-67.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for HNRNPF

Target: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Alternative Name: HNRNPF

Background:

Synonyms: Heterogeneous nuclear ribonucleoprotein F,hnRNP F,Nucleolin-like protein mcs94-1,Heterogeneous nuclear ribonucleoprotein F, N-terminally processed,HNRNPF,HNRPF,

Tissue Specificity: Expressed ubiquitously.

Background: Heterogeneous nuclear ribonucleoprotein F is a protein that in humans is encoded by the HNRNPF gene. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Molecular Weight: 46 kDa

Gene ID: 3185

UniProt: P52597