HPRT1 antibody (AA 2-110)

Catalog No. ABIN4886626

Product Details anti-HPRT1 Antibody

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Binding Specificity: AA 2-110

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HPRT1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: Rabbit IgG polyclonal antibody for Hypoxanthine-guanine phosphoribosyltransferase(HPRT1) detection. Tested with WB in Human,Mouse,Rat.

Cross-Reactivity (Details): No cross reactivity with other proteins.

Characteristics: Rabbit IgG polyclonal antibody for Hypoxanthine-guanine phosphoribosyltransferase(HPRT1) detection. Tested with WB in Human,Mouse,Rat.
Gene Name: hypoxanthine phosphoribosyltransferase 1
Protein Name: Hypoxanthine-guanine phosphoribosyltransferase

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human HPRT recombinant protein (Position: A2-S110). Human HPRT shares 98.1% and 96.3% amino acid (aa) sequence identity with mouse and rat HPRT, respectively.

Isotype: IgG

Application Details

Application Notes: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
Notes: Tested Species: Species with positive results.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Target Details for HPRT1

Target: HPRT1 (Hypoxanthine phosphoribosyltransferase 1 (HPRT1))

Alternative Name: HPRT1 (HPRT1 Products)

Synonyms: HGPRT antibody, HPRT antibody, C81579 antibody, HPGRT antibody, Hprt1 antibody, Hgprtase antibody, Hprt antibody, id:ibd1344 antibody, id:ibd5108 antibody, wu:fc10g09 antibody, zgc:56221 antibody, zgc:86608 antibody, hgprt antibody, hprt antibody, prtfdc1 antibody, hprt1 antibody, hprt1l antibody, zgc:55561 antibody, zgc:86771 antibody, hypoxanthine phosphoribosyltransferase 1 antibody, hypoxanthine guanine phosphoribosyl transferase antibody, hypoxanthine phosphoribosyltransferase 1 L homeolog antibody, phosphoribosyl transferase domain containing 1 antibody, HPRT1 antibody, Hprt antibody, Hprt1 antibody, hprt1 antibody, hprt1.L antibody, prtfdc1 antibody

Background: Hypoxanthine-guanine phosphoribosyltransferase (HGPRT) is an enzyme encoded in humans by the HPRT1 gene. The protein encoded by this gene is a transferase, which catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate. This enzyme plays a central role in the generation of purine nucleotides through the purine salvage pathway. Mutations in this gene result in Lesch-Nyhan syndrome or gout.

Synonyms: HGPRT | HGPRTase | HPRT 1 | HPRT1 | P00492

Gene ID: 3251

UniProt: P00492

Pathways: Ribonucleoside Biosynthetic Process