IL7R antibody (C-Term)

Catalog No. ABIN4886632

This Rabbit Polyclonal antibody specifically detects IL7R in FACS and WB. It exhibits reactivity toward Human and Rat.

Quick Overview for IL7R antibody (C-Term) (ABIN4886632)

Target: IL7R (Interleukin 7 Receptor (IL7R))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL7R antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB)

Product Details anti-IL7R Antibody

Binding Specificity: AA 278-315, C-Term

Purpose: Anti-IL7R alpha Antibody Picoband®

Sequence: DHKKTLEHLC KKPRKNLNVS FNPESFLDCQ IHRVDDIQ

Cross-Reactivity (Details): No cross-reactivity with other proteins

Characteristics: Anti-IL7R alpha Antibody Picoband® (ABIN4886632). Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human IL7R alpha, different from the related mouse sequence by nine amino acids.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
1. "Entrez Gene: IL7R interleukin 7 receptor". 2. Kroemer RT, Richards WG (December 1996). "Homology modeling study of the human interleukin-7 receptor complex".Protein Eng. 9 (12): 1135-42. 3. O'Doherty C, Alloza I, Rooney M, Vandenbroeck K (November 2009). "IL7RA polymorphisms and chronic inflammatory arthropathies". Tissue Antigens 74 (5): 429-31.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for IL7R

Target: IL7R (Interleukin 7 Receptor (IL7R))

Alternative Name: IL7R

Background:

Synonyms: Interleukin-7 receptor subunit alpha,IL-7 receptor subunit alpha,IL-7R subunit alpha,IL-7R-alpha,IL-7RA,CDw127,CD127,IL7R,

Tissue Specificity: Predominantly expressed in kidney and brain. Detected at much lower levels in heart and skeletal muscle. .

Background: The interleukin-7 receptor, also known as IL7R alpha, is a protein found on the surface of cells. It is mapped to 5p13. Interleukin-7 receptor has been shown to play a critical role in the development of immune cells called lymphocytes - specifically in a process known as V (D)J recombination. This protein is also found to control the accessibility of a region of the genome that contains the T-cell receptor gamma gene, by STAT5 and histone acetylation. Knockout studies in mice suggest that blocking apoptosis is an essential function of this protein during differentiation and activation of T lymphocytes. Functional defects in this protein may be associated with the pathogenesis of severe combined immunodeficiency (SCID).

Molecular Weight: 70 kDa

Gene ID: 3575

UniProt: P16871

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Regulation of Cell Size