IRF9 antibody (AA 1-110)

Catalog No. ABIN4886637

This anti-IRF9 antibody is a Rabbit Polyclonal antibody detecting IRF9 in WB. Suitable for Human.

Quick Overview for IRF9 antibody (AA 1-110) (ABIN4886637)

Target: IRF9 (Interferon Regulatory Factor 9 (IRF9))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRF9 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-IRF9 Antibody

Binding Specificity: AA 1-110

Purpose: Anti-Interferon regulatory factor 9/IRF9 Antibody Picoband®

Cross-Reactivity (Details): No cross-reactivity with other proteins.

Characteristics: Anti-Interferon regulatory factor 9/IRF9 Antibody Picoband® (ABIN4886637). Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogen: E. coli-derived human IRF9 recombinant protein (Position: M1-K110). Human IRF9 shares 87.3% amino acid (aa) sequence identity with mouse IRF9.

Isotype: IgG

Application Details

Application Notes: Western blot, 0.1-0.5 μg/mL, Human
1. Fu, X.-Y., Kessler, D. S., Veals, S. A., Levy, D. E., Darnell, J. E., Jr. ISGF3, the transcriptional activator induced by interferon alpha consists of multiple interacting polypeptide chains. Proc. Nat. Acad. Sci. 87: 8555-8559, 1990. 2. Levy, D. E., Kessler, D. S., Pine, R., Darnell, J. E., Jr. Cytoplasmic activation of ISGF3, the positive regulator of interferon-alpha-stimulated transcription, reconstituted in vitro. 3. Suhara, W., Yoneyama, M., Yonekawa, H., Fujita, T. Structure of mouse interferon stimulated gene factor 3 gamma (ISGF3 gamma/p48) cDNA and chromosomal localization of the gene. J. Biochem. 119: 231-234, 1996.

Comment:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Target Details for IRF9

Target: IRF9 (Interferon Regulatory Factor 9 (IRF9))

Alternative Name: IRF9

Background:

Synonyms: Interferon regulatory factor 9,IRF-9,IFN-alpha-responsive transcription factor subunit,ISGF3 p48 subunit,Interferon-stimulated gene factor 3 gamma,ISGF-3 gamma,Transcriptional regulator ISGF3 subunit gamma,IRF9,ISGF3G,

Tissue Specificity: Leukocytes.

Background: Interferon regulatory factor 9 is a protein that in humans is encoded by the IRF9 gene, previously known as ISGF3G. It is mapped to 14q1. IRF9 is a 48 kDa member of the IRF family of proteins. It is widely expressed, and serves as a component of the ISGF3 complex. In addition, IRF9 is an interferon-dependent, positive-acting transcription factor that is cytoplasmically activated, possibly through interaction with the interferon receptor. It associates with activated STAT1 and STAT2 to form an ISGF3 complex that is translocated into the nucleus.

Molecular Weight: 44 kDa

Gene ID: 10379

UniProt: Q00978

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, Hepatitis C